Effect of different storage media on elemental analysis and microhardness of cervical cavity margins restored with a bioactive material
Hoda Saleh Ismail, Brian Ray Morrow, Ashraf Ibrahim Ali, Rabab Elsayed Elaraby Mehesen, Salah Hasab Mahmoud, Franklin Garcia-Godoy,
소속 상세정보
( Hoda Saleh Ismail ) -
( Brian Ray Morrow ) -
( Ashraf Ibrahim Ali ) -
( Rabab Elsayed Elaraby Mehesen ) -
( Salah Hasab Mahmoud ) -
( Franklin Garcia-Godoy ) -
Abstract
Objectives: This study aimed to investigate the elemental analysis and microhardness of a bioactive material (Activa) and marginal tooth structure after storage in different media.
Materials and Methods: Fifteen teeth received cervical restorations with occlusal enamel and gingival dentin margins using the tested material bonded with a universal adhesive, 5 of them on the 4 axial surfaces and the other 10 on only the 2 proximal surfaces. The first 5 teeth were sectioned into 4 restorations each, then stored in 4 different media; deionized water, Dulbecco's phosphate buffered saline (DPBS), Tris buffer, and saliva. The storage period for deionized water was 24 hours while it was 3 months for the other media. Each part was analyzed by scanning electron microscopy-energy dispersive spectroscopy (SEM-EDS) analysis for different substrates/distances and the wt% of calcium, phosphorus, silica, and fluoride were calculated. The other 10 teeth were sectioned across the restoration, stored in either Tris buffer or saliva for 24 hours or 3 months, and were evaluated for microhardness of different substrates/areas. Data were analyzed using analysis of variance and Tukey’s post hoc test.
Results: Enamel and dentin interfaces in the DPBS group exhibited a significant increase in calcium and phosphorus wt%. Both silica and fluoride significantly increased in tooth structure up to a distance of 75 μm in the 3-month-media groups than the immediate group. Storage media did not affect the microhardness values.
Conclusions: SEM-EDS analysis suggests an ion movement between Activa and tooth structure through a universal adhesive while stored in DPBS.
키워드
Bioactivity; Marginal tooth structure; Microhardness; SEM-EDS
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